the flow chart shows details for an Australian diagnostic guideline for overseas acquired Lyme disease and is described below.
Flow chart for an Australian diagnostic guideline for overseas acquired Lyme disease:
1.	If a patient presents with history of travel through an endemic area, tick bite and relevant symptoms consider Lyme disease in the differential diagnoses. 

Refer for testing - Immunoassay non-reactive or Immunoassay reactive Immunoblot non-reactive or Immunoassay reactive Immunoblot reactive.

If Immunoassay non-reactive or Immunoassay reactive Immunoblot non-reactive not Lyme disease.

If Immunoassay reactive Immunoblot reactive it is Lyme disease and need treatment. Suggest seek advice from infectious diseases physician.

2.	If patient presents with no history of travel through an endemic area, but with tick bite and systemic symptoms, e.g., fever.

Refer for testing for tick borne infections in Australia (Lyme disease is not an Australian tick borne infection) (With advice from specialist microbiologist).

If Reactive results treat for specific infections with advice from infectious diseases physician.

If Non-reactive results consider an alternative diagnosis with advice from infectious diseases physician.