Australian Group on Antimicrobial Resistance Australian Enterobacteriaceae Sepsis Outcome Programme annual report, 2014

Results of the Australian Group on Antimicrobial Resistance surveillance program of 2014 for Enterobacteriacae isolated from blood stream infections are presented. Low but growing rates of extended-spectrum ß-lactamases are now being observed, and carbapenemase-producing strains, although rare, are now being observed. Resistance to fluoroquinolones remained steady, but multi-drug resistance among E. coli and K. pneumoniae was more common than in 2013.

Page last updated: 30 June 2016

Jan M Bell, John D Turnidge, Geoffrey W Coombs, Denise A Daley, Thomas Gottlieb, Jenny Robson, Narelle George


The Australian Group on Antimicrobial Resistance performs regular period-prevalence studies to monitor changes in antimicrobial resistance in selected enteric Gram-negative pathogens. The 2014 survey was the second year to focus on blood stream infections. During 2014, 5,798 Enterobacteriaceae species isolates were tested using commercial automated methods (Vitek 2, BioMérieux; Phoenix, BD) and results were analysed using the Clinical and Laboratory Standards Institute (CLSI) and European Committee on Antimicrobial Susceptibility Testing (EUCAST) breakpoints (January 2015). Of the key resistances, non-susceptibility to the third-generation cephalosporin, ceftriaxone, was found in 9.0%/9.0% of Escherichia coli (CLSI/EUCAST criteria) and 7.8%/7.8% of Klebsiella pneumoniae, and 8.0%/8.0% K. oxytoca. Non-susceptibility rates to ciprofloxacin were 10.4%/11.6% for E. coli, 5.0%/7.7% for K. pneumoniae, 0.4%/0.4% for K. oxytoca, and 3.5%/6.5% in Enterobacter cloacae. Resistance rates to piperacillin-tazobactam were 3.2%/6.8%, 4.8%/7.2%, 11.1%/11.5%, and 19.0%/24.7% for the same 4 species respectively. Fourteen isolates were shown to harbour a carbapenemase gene, 7 blaIMP-4, 3 blaKPC-2, 3 blaVIM-1, 1 blaNDM-4, and 1 blaOXA-181-lke .Commun Dis Intell 2016;40(2):E229–E235.

Keywords: antibiotic resistance; bacteraemia; gram-negative; Escherichia coli; Enterobacter; Klebsiella

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Emerging resistance in common pathogenic members of the Enterobacteriaceae is a world-wide phenomenon, and presents therapeutic problems for practitioners in both the community and in hospital practice. The Australian Group on Antimicrobial Resistance (AGAR) commenced surveillance of the key Gram-negative pathogens, Escherichia coli and Klebsiella species in 1992. Surveys have been conducted biennially until 2008 when annual surveys commenced, alternating between community– and hospital-onset infections.1 In 2004, another genus of Gram-negative pathogens in which resistance can be of clinical importance, Enterobacter species, was added. E. coli is the most common cause of community-onset urinary tract infection, while Klebsiella species are less common but are known to harbour important resistances. Enterobacter species are less common in the community, but of high importance due to intrinsic resistance to first-line antimicrobials in the community. Taken together, the three groups of species surveyed are considered to be valuable sentinels for multi-resistance and emerging resistance in enteric Gram-negative bacilli. In 2013 AGAR commenced the Enterobacteriaceae Sepsis Outcome Programme (EnSOP), which focused on the collection of resistance and some demographic data on all isolates prospectively from patients with bacteraemia. The 2014 survey was the second EnSOP survey.

Resistances of particular interest include resistance to ß-lactams due to ß-lactamases, especially extended-spectrum ß-lactamases, which inactivate the third-generation cephalosporins that are normally considered reserve antimicrobials. Other resistances of interest are to agents important for treatment of these serious infections, such as gentamicin; and resistance to reserve agents such as ciprofloxacin and meropenem.

The objectives of the 2014 surveillance program were to:

  • monitor resistance in Enterobacteriaceae isolated from blood;
  • examine the extent of co-resistance and multi-resistance; and
  • detect emerging resistance to newer last-line agents such as carbapenems.

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Study design

From 1 January to 31 December 2014, 26 institutions across Australia collected either all or up to 200 isolates from different patient episodes of bacteraemia.

Species identification

Isolates were identified using the routine method for each institution; Vitek®, Phoenix™ Automated Microbiology System, or where available, mass spectrometry (MALDI-TOF).

Susceptibility testing

Testing was performed by 2 commercial semi-automated methods, Vitek 2 (BioMérieux) or Phoenix (BD), which are calibrated to the ISO reference standard method of broth microdilution. Commercially available Vitek AST-N246, or Phoenix NMIC-203 cards were utilised by all participants throughout the survey period. The Clinical and Laboratory Standards Institute (CLSI) M1002 and European Committee on Antimicrobial Susceptibility Testing (EUCAST) v5.03 breakpoints from January 2015 have been employed in the analysis. For analysis of cefazolin, breakpoints of ≤ 4 for susceptible, and ≥ 8 for resistant were applied due to the restricted minimum inhibitory concentration (MIC) range available on the commercial cards, recognising that the January 2015 breakpoint is actually susceptible ≤ 2 mg/L.

Molecular confirmation of resistances

E. coli and Klebsiella isolates with ceftazidime or ceftriaxone MIC > 1 mg/L, or cefoxitin MIC > 8 mg/L; Enterobacter spp. with cefepime MIC > 1 mg/L; all isolates with ciprofloxacin MIC > 0.25 mg/L; all isolates with meropenem MIC > 0.25 mg/L; and all isolates with amikacin MIC > 32 mg/L were referred to a central laboratory (SA Pathology) for molecular confirmation of resistance.

All referred isolates were screened for the presence of the blaTEM, and blaSHV genes using a real-time polymerase chain reaction (PCR) platform (LC-480) and published primers.4,5 A multiplex real-time TaqMan PCR was used to detect CTX-M-type genes.6 Strains were probed for plasmid-borne AmpC enzymes using the method described by Pérez-Pérez and Hanson,7 and subjected to molecular tests for MBL (blaVIM, blaIMP, and blaNDM), blaKPC, and blaOXA-48-like genes using real-time PCR.8,9 Known plasmid mediated quinolone resistance mechanisms (Qnr, efflux (qepA, oqxAB), and aac(6’)-Ib-cr) were examined by PCR on all referred isolates with ciprofloxacin MIC > 0.25 mg/L using published methods.10,11 All E. coli were examined for presence of the O25b-ST131 clone and its H30- and H30-Rx subclones.12–14

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The species isolated, and the numbers of each are listed in Table 1. Three genera, Escherichia spp., Klebsiella spp. and Enterobacter spp. contributed 87.6% of all isolates. Major resistances and non-susceptibilities for the top 6 ranked species are listed in Table 2. Non-susceptibility, (which includes both intermediately resistant and resistant strains), has been included for some agents because these figures provide information about important emerging acquired resistances. Multiple acquired resistances by species are shown in Table 3. Multi-resistance was detected in 13.4% of E. coli isolates, 9.7% of K. pneumoniae, and 12.1% of Ent. cloacae. A more detailed breakdown of resistances and non-susceptibilities by state and territory is provided in the online report from the group (

Table 1: Species tested
Species Total %
Escherichia coli 3,493 60.2
Klebsiella pneumoniae 877 15.1
Enterobacter cloacae 343 5.9
Klebsiella oxytoca 226 3.9
Proteus mirabilis 187 3.2
Serratia marcescens 136 2.3
Enterobacter aerogenes 105 1.8
Salmonella species (non Typhi) 94 1.6
Morganella morganii 57 1.0
Citrobacter freundii 53 0.9
Citrobacter koseri 50 0.9
Salmonella Typhi/Paratyphi 26 0.4
Enterobacter asburiae 16 0.3
Raoultella ornithinolytica 15 0.3
Pantoea species 12 0.2
Pantoea agglomerans 12 0.2
Enterobacter species 11 0.2
Providencia stuartii 10 0.2
Other species (n=27) 75 1.3
Total 5,798

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Table 2: Non-susceptibility and resistance rates for the top 6 ranked species tested
    Escherichia coli
Klebsiella pneumoniae
Klebsiella oxytoca
Enterobacter cloacae
Proteus mirabilis
Serratia marcescens

* R = resistant, I = intermediate, NS = non-susceptible (intermediate + resistant), using criteria as published by the Clinical and Laboratory Standards Institute (CLSI) [2014] and European Committee on Antimicrobial Susceptibility Testing (EUCAST) [2014].

† Considered largely intrinsically resistant due to natural ß-lactamases; – no intermediate category; / no breakpoints defined

‡ For EUCAST interpretation, the clavulanate is fixed at 2 mg/L, rather than a 2:1 ratio used in CLSI guidelines. As all cards used have a 2:1 ratio of clavulanate no EUCAST category has been applied.

Ampicillin I 1.8 0.5
R 50.1 51.9 16.8 17.3
Amoxycillinclavulanate I 12.7 5.1 4.4 8.7
R 8.2 5.3 8.8 1.6
Ticarcillin-clavulanate R 9.4 19.3 7.2 11.4 10.4 12.2 24.4 29.7 1.1 1.7 0.0 2.2
Piperacillintazobactam R 3.2 6.8 4.8 7.2 11.1 11.5 19.0 24.7 1.1 1.6 0.0 0.0
Cefazolin R 20.5 / 13.0 / 66.0 / 26.5 /
Cefoxitin R 3.8 / 6.2 / 0.9 / 0.0 /
Ceftriaxone NS 9.0 9.0 7.8 7.8 8.0 8.0 27.6 27.6 0.5 0.5 2.9 2.9
Ceftazidime NS 4.4 8.0 6.1 8.0 0.4 0.4 24.6 27.0 0.0 0.0 2.2 2.2
Cefepime NS 3.3 6.4 3.7 6.1 0.0 0.0 4.1 14.4 1.1 1.1 1.5 2.2
Meropenem NS 0.1 0.1 1.1 1.0 0.0 0.0 2.9 2.3 0.5 0.5 0.7 0.7
Ciprofloxacin NS 10.4 11.6 5.0 7.6 0.4 0.4 3.5 6.5 2.7 3.2 1.5 3.7
Norfloxacin NS 10.4 18.2 4.5 13.7 0.0 1.8 3.2 13.5 3.2 5.4 0.7 3.7
Gentamicin NS 7.5 8.0 5.5 6.1 1.3 1.8 6.7 7.6 1.6 2.2 1.5 1.5
Trimethoprim R 29.2 29.4 15.5 16.6 4.0 4.4 19.1 19.1 21.7 22.3 1.5 2.2
Nitrofurantoin NS 6.6 1.6 88.8 / 41.6 / 72.6 /

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Table 3: Multiple acquired resistances, by species
Species Total Number of acquired resistances (CLSI breakpoints)
Non-multi-resistant Multi-resistant
0 1 2 3 Cumulative % 4 5 6 7 8 9 10 11 12 13 Cumulative %

* Antibiotics included: amoxycillin-clavulanate, piperacillin-tazobactam, cefazolin, cefoxitin, ceftriaxone, ceftazidime, cefepime, gentamicin, amikacin, ciprofloxacin, nitrofurantoin, trimethoprim, meropenem;
Antibiotics excluded: ampicillin (intrinsic resistance), ticarcillin-clavulanate, tobramycin, norfloxacin, nalidixic acid, sulfamethoxazole-trimethoprim (high correlation with antibiotics in the included list)

† Antibiotics included: piperacillin-tazobactam, ceftriaxone, ceftazidime, cefepime, gentamicin, amikacin, ciprofloxacin, nitrofurantoin, trimethoprim, meropenem
Antibiotics excluded: ampicillin, amoxycillin-clavulanate, cefazolin, and cefoxitin, (all four due to intrinsic resistance); also excluded were ticarcillin-clavulanate, tobramycin, norfloxacin, nalidixic acid, sulfamethoxazole-trimethoprim (high correlation with antibiotics in the included list).

Escherichia coli 2,958 1,324 506 505 226 133 109 71 44 22 11 6 0 0 0
% 44.8 17.1 17.1 7.6 86.6 4.5 3.7 2.4 1.5 0.7 0.4 0.2 0.0 0.0 0.0 13.4
Klebsiella pneumoniae* 746 417 192 51 14 17 15 11 6 6 5 4 2 6
% 55.9 25.7 6.8 1.9 90.3 2.3 2.0 1.5 0.8 0.8 0.7 0.5 0.3 0.8 9.7
Enterococcus cloacae 330 163 66 24 37 20 12 5 1 2
% 49.4 20.0 7.3 11.2 87.9 6.1 3.6 1.5 0.3 0.6 12.1
Proteus mirabilis 148 3 87 35 13 7 2 0 0 0 0 1
% 2.0 58.8 23.6 8.8 93.2 4.7 1.4 0.0 0.0 0.0 0.0 0.7 6.8
Serratia marcescens 98 1 94 0 1 2
% 1.0 95.9 0.0 1.0 98.0 2.0 2.0
Klebsiella oxytoca* 197 66 98 14 10 7 2
% 33.5 49.7 7.1 5.1 95.4 3.6 1.0 4.6
Enterococcus aerogenes 104 36 31 12 18 5 1 1
% 34.6 29.8 11.5 17.3 93.3 4.8 1.0 1.0 6.7
Salmonella spp. (non-Typhi) 71 57 8 4 2
% 80.3 11.3 5.6 2.8 100.0 0.0

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Escherichia coli

Moderately high levels of resistance to ampicillin (and therefore amoxycillin) were maintained (50.1%/51.9%, CLSI/EUCAST criteria), with lower rates for amoxycillin-clavulanate (12.7%/- intermediate, 8.2%/20.9% resistant). Non-susceptibility to third-generation cephalosporins was low (ceftriaxone 9.0%/9.0%, ceftazidime 4.4%/8.0%). Moderate levels of resistance were detected to cefazolin (20.5%/-) and trimethoprim (29.2%/29.4%). Ciprofloxacin non-susceptibility was found in 10.4%/11.6% of E. coli isolates. Resistance to ticarcillin-clavulanate (9.4%/19.3%), gentamicin (7.3%/7.5%), piperacillin-tazobactam (3.2%/6.8%), and cefepime (1.6%/2.9%) were low. Nine isolates had elevated meropenem MICs (≥ 0.5 mg/L). For the extended-spectrum ß-lactamase (ESBL)-producing strains, ciprofloxacin and gentamicin resistance was found in 51.6%/51.6% and 33.8%/34.1% respectively.

In line with international trends among community strains of E. coli, most of the strains with ESBL genes harboured genes of the CTX-M type (222/272 = 82%). Over 60% of E. coli with CTX-M group 1 types were found to belong to sequence type 131 (O25b-ST131). ST131 accounted for 68% of E. coli ESBL phenotypes that were ciprofloxacin resistant (MIC > 1 mg/L), and only 6% of ciprofloxacin susceptible ESBL phenotypes. Ninety-one per cent and 41% of O25b-ST131 were associated with the H30 and H30-Rx subclones, respectively, with their reported association with more antibiotic resistances and greater virulence potential.13

Klebsiella pneumoniae

K. pneumoniae showed slightly higher levels of resistance to piperacillin-tazobactam and ceftazidime compared with E. coli, but lower rates of resistance to amoxycillin-clavulanate, ticarcillin-clavulanate, cefazolin, ceftriaxone ciprofloxacin, gentamicin, and trimethoprim. Thirteen K. pneumoniae isolates had elevated meropenem MICs. ESBLs were present in 61 of 69 (88%) presumptively ESBL-positive isolates of K. pneumoniae, 47 (77%) of which proved to be of the CTX-M type.

Enterobacter species

Acquired resistance was common to ticarcillin-clavulanate (24.4%/29.7% and 26.7%/40.6%), piperacillin-tazobactam (19.0%/24.7% and 23.1%/31.7%), ceftriaxone (27.3%/27.3% and 37.5%/37.5%), ceftazidime (24.3%/24.6% and 29.8%/33.7%) and trimethoprim (19.1%/19.1% and 1.9%/1.9%) for Ent. cloacae and Ent. aerogenes, respectively. Cefepime, ciprofloxacin, and gentamicin resistance were all less than 10%. Seventeen of 45 Ent. cloacae tested for ESBL based on a suspicious phenotype, harboured ESBL-encoding genes. Eighteen Ent. cloacae strains had elevated meropenem MICs.

Carbapenemase resistance

Overall, 14 isolates (14 patients) in 9 institutions from 5 states or territories were found to harbour a carbapenemase gene. BlaIMP-4 was detected in E. cloacae (5) and K. pneumoniae (2); blaKPC-2 was detected in 3 K. pneumoniae isolates from 1 institution; blaVIM-1 was detected in 2 K. pneumoniae; blaNDM-4 in 1 E. coli, and blaOXA-181-like in 1 K. pneumoniae.

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AGAR has been tracking resistance in sentinel enteric Gram-negative bacteria since 1992. From 2008, surveillance was segregated into hospital- versus community-onset infections. The last year of hospital-onset only surveillance was 2011.15 In 2013, the first survey of antimicrobial resistance among Enterobacteriaceae isolates from bacteraemic patients throughout Australia was conducted using an approach similar to that conducted by the European EARS-Net program. The 2014 survey was the second survey conducted of antimicrobial resistance among Enterobacteriaceae isolates from bacteraemic patients throughout Australia.

CTX-M-producing E. coli and Klebsiella species and gentamicin- and ciprofloxacin-resistant E. coli continued to be a problem in patients with bacteraemia. Of concern is the high proportion of E. coli that belong to the ST131 H30-Rx subclone, and its reported association with more antibiotic resistance and greater virulence potential.13 Carbapenem resistance attributable to acquired carbapenemases are still uncommon in patients with bacteraemia in Australia, although 5 different types (IMP, KPC, VIM, NDM and OXA-181-like) were detected from 9 of the participating institutions. Compared with many other countries in our region, resistance rates in Australian Gram-negative bacteria are still relatively low,16 but similar to those observed in 2014 in many Western European countries.17

Multi-resistance is being increasingly observed, especially in E. coli and E. cloacae, both of which have multi-resistance rates (as defined by AGAR) above 10%. This is likely to drive more broad-spectrum antibiotic use, and increase the resistance selection pressure for important reserve classes, especially the carbapenemases.

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AGAR participants

Australian Capital Territory

Peter Collignon and Susan Bradbury, The Canberra Hospital

New South Wales

Thomas Gottlieb and Graham Robertson, Concord Hospital

John Ferguson and Jack (Ian) Winney, John Hunter Hospital

James Branley and Donna Barbaro, Nepean Hospital

George Kotsiou and Peter Huntington, Royal North Shore Hospital

David Mitchell and Lee Thomas, Westmead Hospital

Northern Territory

Rob Baird and Jann Hennessy, Royal Darwin Hospital


Enzo Binotto and Bronwyn Thomsett, Pathology Queensland Cairns Base Hospital

Graeme Nimmo and Narelle George, Pathology Queensland Central Laboratory

Petra Derrington and Sharon Dal-Cin, Pathology Queensland Gold Coast Hospital

Robert Horvath, Pathology Queensland Prince Charles Hospital

Naomi Runnegar and Joel Douglas, Pathology Queensland Princess Alexandra Hospital

Jenny Robson and Georgia Peachey, Sullivan Nicolaides Pathology

South Australia

Kelly Papanoum and Nicholas Wells, SA Pathology (Flinders Medical Centre)

Morgyn Warner and Kija Smith, SA Pathology (Royal Adelaide Hospital)

John Turnidge and Jan Bell, SA Pathology (Women’s and Children’s Hospital)

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Louise Cooley and Rob Peterson, Royal Hobart Hospital


Denis Spelman, Amanda Dennison and Christopher Lee, Alfred Hospital

Benjamin Howden and Peter Ward, Austin Hospital

Tony Korman and Despina Kotsanas, Southern Health, Monash Medical Centre

Andrew Daley and Gena Gonis, Royal Women’s Hospital

Mary Jo Waters and Linda Joyce, St Vincent’s Hospital

Western Australia

David McGechie and Rebecca Wake, PathWest Laboratory Medicine - WA, Fremantle Hospital

Ronan Murray and Barbara Henderson, PathWest Laboratory Medicine – WA, Queen Elizabeth II Hospital

Owen Robinson, Denise Daley and Geoffrey Coombs, PathWest Laboratory Medicine – WA, Royal Perth Hospital

Sudha Pottumarthy-Boddu and Fay Kappler, St John of God Pathology

Author details

Prof John D Turnidge1

Ms Jan M Bell2

A/Prof Geoffrey W Coombs3,4

Ms Denise A Daley5

A /Prof Thomas Gottlieb6

Dr Jenny Robson7

Ms Narelle George8

  1. Departments of Pathology, Paediatrics and Molecular and Biomedical Sciences, University of Adelaide, Adelaide, South Australia
  2. Microbiology and Infectious Diseases Directorate, SA Pathology, Adelaide, South Australia
  3. Australian Collaborating Centre for Enterococcus and Staphylococcus Species (ACCESS) Typing and Research, School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia
  4. Department of Microbiology and Infectious Diseases, PathWest Laboratory Medicine-WA, Fiona Stanley Hospital, Murdoch, Western Australia
  5. Australian Group on Antimicrobial Resistance, Fiona Stanley Hospital, Murdoch, Western Australia
  6. Concord Hospital, Concord, New South Wales
  7. Sullivan Nicolaides Pathology, Queensland
  8. Microbiology, Pathology Queensland, Royal Brisbane and Women’s Hospital, Herston, Queensland

Corresponding author: Prof John D Turnidge, Departments of Pathology, Paediatrics and Molecular and Biomedical Sciences, University of Adelaide, ADELAIDE SA 8000. Telephone: +61 417 811 552. Email:

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